The answer to the question of the title is YES, OF COURSE! Why wouldn't it? Well, the reason of this question is the following: you may already know that CAPICOM has been deprecated on Windows 7 (CAPICOM support on Windows 7). It was going to be deprecated when Vista came out, but it didn't (CAPICOM support on Windows Vista). And why wasn't it deprecated on Vista? Because a tool like SignTool depended on CAPICOM.
So if SignTool depends on CAPICOM, will it work on Windows 7? Yes, because Signtool has been updated on Windows 7 and it does not use CAPICOM anymore.
Note: remember that Win7 version of SignTool comes with Microsoft Windows SDK for Windows 7 and .NET Framework 3.5 SP1.
I hope this helps.
Alex (Alejandro Campos Magencio)